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Laboratory Exercises in Microbiology: Discovering the Unseen World Through Hands-On Investigation

(3 reviews)

Susan McLaughlin, Queensborough Community College

Joan Petersen, Queensborough Community College

Pub Date: 2016

Publisher: CUNY Academic Works

Language: English

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Reviewed by Becky Buckley, Affiliate Professor, Metropolitan State University of Denver on 6/12/19

The lab manual covers most core aspects of the microbial world very well: bacteria, fungi, and protists, but does not include any experiments with the viruses. A simple exercise with bacteriophage T4 and Escherichia coli could be added to... read more


Reviewed by Sharon Cisneros, Adjunct, Morton College on 1/14/19

This text provides a solid over-view of microbiology concepts that can be used in a general microbiology course. It provides an appendix in the back of the book, but does not provide a glossary of terms. It does, however, give definitions of... read more


Reviewed by Robert Beatty, Senior Lecturer, University of California, Berkeley on 2/1/18

This is excellent lab manual for introductory microbiology. The book contains wonderful illustrations and provides numerous exercises and hands-on activities. The text is easy to read and well-organized into a thorough overview of clinical... read more


Table of Contents

  • Lab 1. Introduction to Microscopy and Diversity of Cell Types
  • Lab 2. Introduction to Aseptic Techniques and Growth Media
  • Lab 3. Preparation of Bacterial Smears and Introduction to Staining
  • Lab 4. Acid fast and Endospore Staining
  • Lab 5. Metabolic Activities of Bacteria
  • Lab 6. Dichotomous Keys
  • Lab 7. The Effect of Physical Factors on Microbial Growth
  • Lab 8. Chemical Control of Microbial Growth—Disinfectants and Antibiotics
  • Lab 9. The Microbiology of Milk and Food
  • Lab 10. The Eukaryotes
  • Lab 11. Clinical Microbiology I; Anaerobic pathogens; Vectors of Infectious Disease
  • Lab 12. Clinical Microbiology II—Immunology and the Biolog System
  • Lab 13. Putting it all Together: Case Studies in Microbiology
  • Appendix I. Information About Lab Practical Exams
  • Appendix II. Scientific Notation and Serial Dilution
  • Appendix III. Introduction to Micropipetting

About the Book

The exercises in this laboratory manual are designed to engage students in hand-on activities that reinforce their understanding of the microbial world. Topics covered include: staining and microscopy, metabolic testing, physical and chemical control of microorganisms, and immunology. The target audience is primarily students preparing for a career in the health sciences, however many of the topics would be appropriate for a general microbiology course as well.

About the Contributors


Susan McLaughlin is a Professor in the Biology department at Queensborough Community College, Bayside, NY.

Joan Petersen Professor in the Science department at Queensborough Community College, Bayside, NY