Read more about Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual

Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual

(2 reviews)

Ryan Mehl, Oregon State University

Kari van Zee, Oregon State University

Kelsey Kean, Oregon State University

Copyright Year: 2019

Publisher: Oregon State University

Language: English

Formats Available

Conditions of Use

Attribution-NonCommercial Attribution-NonCommercial
CC BY-NC

Reviews

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Reviewed by Caylin Murray, Genetics Lab Coordinator and Instructor, James Madison University on 11/30/20

This lab manual is designed for graduate students but it seems it could easily be designed to work as a CURE at a university with necessary resources. This laboratory experiment seems to be designed as a semester long unit. The project does a... read more

Reviewed by Karina Barseguian, Adjunct Faculty,, Worcester State University on 6/29/20

This Lab Manual is for graduate students and may also be used as an experimental framework for independent research. Readers/students must know the principles of molecular biology and biochemistry, and also be familiar with the terminology and... read more

Table of Contents

  •  1. Background
  •  2. Overview of Laboratory Responsibilities Tentative Schedule
  •  3. Objectives for Week 1
  •  4. Concepts for Choosing and Expressing Mutants
  •  5. Objectives for Week 2
  •  6. Concepts for Protein Expression
  •  7. Methods for Protein Expression
  •  8. Concepts for SDS-PAGE
  •  9. Methods for SDS-PAGE
  •  10. Objectives for Week 3
  •  11. Concepts for Protein Purification and Desalting
  •  12. Methods for Protein Purification and Desalting
  •  13. Microfluidizer Lysis Method
  •  14. Objectives for Week 4
  •  15. Methods for Protein Standard Curves and SDS-PAGE
  •  16. Objectives for Weeks 5-9
  •  17. Concepts for Enzyme Assays
  •  18. Methods for Enzyme Assays

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  • About the Book

    Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual

    About the Contributors

    Authors

    Ryan Mehl, Oregon State University

    Kari van Zee, Oregon State University

    Kelsey Kean, Oregon State University