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    General Biology Laboratory Booklet

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    Martilias Farrell, Cheyney University

    Copyright Year:

    Publisher: The Pennsylvania Alliance for Design of Open Textbooks (PA-ADOPT)

    Language: English

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    Table of Contents

    • Note to the Reader
    • Microscopic Life, Laboratory Notebooks and the Scientific Method
    • Ecosystem Observation and Sample Collection
    • Soil Core Analysis
    • Microscopic Analysis
    • Biochemical Analysis of Environmental Samples
    • Enzyme Lab I
    • Enzyme Lab II – Modifiers of enzyme rate
    • Enzyme Lab III – Concentration Effect curve
    • Planaria Locomotor Lab
    • Planaria Regeneration Screen
    • Concentration-Dependent Effects of Screen Hits on Planaria Regeneration
    • Appendix A: Note for the Instructor
    • Appendix B: Lab Notebook Format Entry Guide
    • Appendix C: DIY Imager
    • Appendix D: Using ImageJ To Quantify Images
    • Image Citations

    About the Book

    This laboratory manual provides a diverse collection of experiments designed to immerse students in techniques mirroring those used in professional biomedical research. Students will explore fundamental enzyme kinetics, investigating how varying amylase concentrations and the introduction of chemical modifiers influence starch digestion rates. These labs emphasize quantitative analysis through colorimetric assays and densitometry using ImageJ software. The booklet also features studies with planaria, where students will assess how common substances affect locomotor activity and screen compounds for their impact on the remarkable regenerative abilities of these organisms, including generating concentration-effect curves. Additional activities guide students in using microscopy to observe microscopic life. Throughout these hands-on investigations, students will develop and refine crucial laboratory skills such as preparing serial dilutions, mastering precise pipetting techniques, and maintaining detailed, reproducible laboratory notebooks, thereby building a robust foundation in experimental design, data analysis, and the scientific method.

    About the Contributors

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    Martilias Farrell, Cheyney University

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