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Read more about Biomedical Engineering Lab Manual Volume 2

Biomedical Engineering Lab Manual Volume 2

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Sarah Breen, University of Oklahoma

Copyright Year: 2023

Publisher: SHAREOK

Language: English

Formats Available

Conditions of Use

Attribution-NonCommercial-ShareAlike Attribution-NonCommercial-ShareAlike
CC BY-NC-SA

Table of Contents

Module 1 Introduction to BME3181 & Microscopy

  • Module 1 Lab Procedure

Module 2 Micropipetting & Dilutions

  • Pre Lab-Review
  • Module 2 Lab Procedure
  • Procedure Exercise 1 (Quick Test)
  • Procedure Exercise 2 (Pipetting Challenge)

Module 3 Fluorescence

  • Pre Lab-Review
  • Module 3 Lab Procedure

Module 4 Aseptic Technique & Cell Passaging 

  • Pre Lab-Review
  • Module 4 Lab Procedure
  • Module 4 Post Lab Activity

Module 5 Live/Dead Assay

  • Pre Lab-Review
  • Module 5 Lab Procedure
  • Module 5 Post Lab Activity

Module 6 Primary Cell Isolation

  • Pre Lab-Review
  • Module 6 Lab Procedure

Module 7 dsDNA Quantification Assay

  • Pre Lab-Review
  • Module 7 Lab Procedure
  • Module 7 Post Lab Activity

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About the Book

The lab manual was written as the second installment that coincides with two lab courses taught at the University of Oklahoma (BME3171, BME3181). These courses are designed to provide Biomedical Engineering students with lab skills and experience in biomedical engineering research and clinical techniques. This manual is used with BME3181 Biomedical Engineering Lab 2 and the following wet lab topics are covered in this lab manual; bioimaging, cell culture, tissue engineering, live-dead and DNA assays.

About the Contributors

Author

Dr. Sarah Breen, University of Oklahoma

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