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    Biomedical Engineering Lab Manual Volume 2

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    Sarah Breen, University of Oklahoma

    Copyright Year:

    Publisher: SHAREOK

    Language: English

    Formats Available

    Conditions of Use

    Attribution-NonCommercial-ShareAlike Attribution-NonCommercial-ShareAlike
    CC BY-NC-SA

    Table of Contents

    Module 1 Introduction to BME3181 & Microscopy

    • Module 1 Lab Procedure

    Module 2 Micropipetting & Dilutions

    • Pre Lab-Review
    • Module 2 Lab Procedure
    • Procedure Exercise 1 (Quick Test)
    • Procedure Exercise 2 (Pipetting Challenge)

    Module 3 Fluorescence

    • Pre Lab-Review
    • Module 3 Lab Procedure

    Module 4 Aseptic Technique & Cell Passaging 

    • Pre Lab-Review
    • Module 4 Lab Procedure
    • Module 4 Post Lab Activity

    Module 5 Live/Dead Assay

    • Pre Lab-Review
    • Module 5 Lab Procedure
    • Module 5 Post Lab Activity

    Module 6 Primary Cell Isolation

    • Pre Lab-Review
    • Module 6 Lab Procedure

    Module 7 dsDNA Quantification Assay

    • Pre Lab-Review
    • Module 7 Lab Procedure
    • Module 7 Post Lab Activity

    Ancillary Material

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    About the Book

    The lab manual was written as the second installment that coincides with two lab courses taught at the University of Oklahoma (BME3171, BME3181). These courses are designed to provide Biomedical Engineering students with lab skills and experience in biomedical engineering research and clinical techniques. This manual is used with BME3181 Biomedical Engineering Lab 2 and the following wet lab topics are covered in this lab manual; bioimaging, cell culture, tissue engineering, live-dead and DNA assays.

    About the Contributors

    Author

    Dr. Sarah Breen, University of Oklahoma

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