This lab manual is designed for students enrolled in the second lab course in the introductory biology sequence at Iowa State University. It is a semester-long lab course, required by most life-science-related majors at ISU, and includes activities on the scientific method, molecular and cellular biology, energetics, genetics, plant anatomy and physiology, and animal anatomy and physiology.
This book provides an ecology- and function-oriented approach to understanding the evolution of vertebrate structure. The text has a modular format, such that each module can be used as a stand-alone instructional tool, or grouped together as a comprehensive textbook, making the text versatile for use in courses with diverse structures. The text also includes human-specific modules for each anatomical system, as many small, liberal arts colleges teach comparative and human anatomy in a single course. Detailed, human-specific modules may be added or removed, as needed for individual courses, depending on the scope and learning objectives of the class.
Publisher:
The Pennsylvania Alliance for Design of Open Textbooks (PA-ADOPT)
License:
CC BY-NC-SA
Mycology Laboratory Manual (2025) by Laura Ramos-Sepulveda is an eTextbook designed for undergraduate students to introduce them to mycology. The laboratory manual provides students with foundational knowledge in mycology and hands-on experience with key concepts such as fungal hyphal and yeast growth, asexual and sexual reproduction, fungal identification using morphological and molecular tools, appreciation of mushrooms as food, and molecular transformation of fungi. The final chapter focuses on plant pathology, where students are required to apply scientific thinking to design experiments, troubleshoot, and integrate the skills they developed in the previous chapters.
Publisher:
The Pennsylvania Alliance for Design of Open Textbooks (PA-ADOPT)
License:
CC BY-NC-SA
This work is designed primarily for use as a laboratory manual in college-level Embryology and Developmental Biology courses and as a companion to developmental biology textbooks. It explores several model animal systems and a variety of the scientific approaches that have been used to study them. It includes both classical embryological observations and manipulations, and more modern techniques, including organ culture and the disruption of developmental processes through pharmacology. The experiments described in the lab manual are designed for 2- to 3-hour laboratory periods. The introductory protocols in each chapter can be adapted to Introductory Biology or Cell Biology courses, while the later protocols are more suited to an advanced course in Developmental Biology. The manual also includes extensive illustrations, preparation guides and appendices to aid the instructor in setting up each lab.
Publisher:
The Pennsylvania Alliance for Design of Open Textbooks (PA-ADOPT)
License:
CC0
This laboratory manual provides a diverse collection of experiments designed to immerse students in techniques mirroring those used in professional biomedical research. Students will explore fundamental enzyme kinetics, investigating how varying amylase concentrations and the introduction of chemical modifiers influence starch digestion rates. These labs emphasize quantitative analysis through colorimetric assays and densitometry using ImageJ software. The booklet also features studies with planaria, where students will assess how common substances affect locomotor activity and screen compounds for their impact on the remarkable regenerative abilities of these organisms, including generating concentration-effect curves. Additional activities guide students in using microscopy to observe microscopic life. Throughout these hands-on investigations, students will develop and refine crucial laboratory skills such as preparing serial dilutions, mastering precise pipetting techniques, and maintaining detailed, reproducible laboratory notebooks, thereby building a robust foundation in experimental design, data analysis, and the scientific method.
This book is an introduction to the diversity of structure and function in animals at the tissue and organ system level. The focus of this book is on principles and mechanisms that sustain life and maintain homeostasis, including water balance, gas exchange, acquisition and transport of oxygen and nutrients, temperature regulation, electrical and chemical signal transmission, sensory processing, and locomotion.
This is an intermediate level book aimed at undergraduate and graduate students following human biology programmes who have a basic understanding of renal physiology. It covers the clearance technique, and how it can be used to evaluate renal function in humans. It goes through the different calculations, before giving you an opportunity to have a go at some calculations using real data. It finishes by evaluating the values from a clearance experiment, where the effect of the drug ACTZ was investigated.
Publisher:
The Pennsylvania Alliance for Design of Open Textbooks (PA-ADOPT)
License:
CC BY
This laboratory manual presents a novel series of undergraduate-friendly experiments designed to provide students with a safe, accessible, and comprehensive introduction to virology. The manual is intended for students who already possess foundational knowledge in cell biology, genetics, and molecular biology, and it emphasizes hands-on learning through diverse experimental approaches. Unlike many existing virology laboratory manuals that depend on costly infrastructure such as mammalian cell culture facilities or focus narrowly on a single viral system, this manual was developed specifically for primary undergraduate teaching institutions. The experiments utilize readily available equipment while exposing students to representative viruses that infect bacteria, plants, and animals. Through these investigations, students gain practical experience with modern virological techniques and develop transferable, employable skills relevant to biotechnology, research, and related industries. Collectively, the laboratory exercises aim to strengthen conceptual understanding of viral biology, promote technical competency, and inspire student curiosity about viruses as dynamic and influential biological entities.
This interactive, open-access textbook explores how ocean science, sustainability, policy and community intersect to address key marine challenges, from climate change to food security. It integrates global, national and local Australian perspectives, using multimedia and interactive learning to build ocean literacy and support a sustainable Blue Economy.
Molecular biology, examining the structures and functions of nucleic acids and proteins, is crucial to advancing biological science and improving human life, particularly in agriculture and medicine. Advances in the field can transform a crop to resist disease or uncover clues to fight illness by manipulating DNA. Techniques for molecular biology research and applications have advanced rapidly over the past decades, and undergraduates need hands-on experiences in this vital field of science. This text provides a structured approach to learning and practicing those techniques, covering DNA, RNA, and protein across five modules and is tailored for a one-semester undergraduate lab course. Each module extends 2–5 weeks of lab exercises and features.
