Open Signals and Systems Laboratory Exercises is a collection of lab assignments that have been used in EE 224: Signals and Systems I in the Department of Electrical and Computer Engineering at Iowa State University. These lab exercises have been curated, edited, and presented in a consistent format to improve student learning. This second edition provides a thorough coverage of the MATLAB concepts needed to complete each exercise, and incorporates hardware descriptions and explanations into the exercises that had integrated new lab equipment.